| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.085660 |
| Chromosome: | chromosome 4 |
| Location: | 2977035 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g224867 | (1 of 34) IPR001878 - Zinc finger, CCHC-type | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTCATTGTGATGCGTCAGTAAGCGCATGTG |
| Internal bar code: | GTGGACGCCTTCCGGCGCGGCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1308 |
| LEAP-Seq percent confirming: | 99.6053 |
| LEAP-Seq n confirming: | 12114 |
| LEAP-Seq n nonconfirming: | 48 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCAGGAACTCACTAGCCTGC |
| Suggested primer 2: | CCAAGAAGCAGAAGACGGAC |