| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.085670 |
| Chromosome: | chromosome 17 |
| Location: | 4903410 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g734400 | CUL2,CUL3 | (1 of 1) K03347 - cullin 1 (CUL1, CDC53); Ubiquitin ligase SCF complex subunit Cullin | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGAGGAAGGCGTTTACCCCCCTAAGTAACA |
| Internal bar code: | CCGGCCTCACCTTAATATGACC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 627 |
| LEAP-Seq percent confirming: | 97.1808 |
| LEAP-Seq n confirming: | 586 |
| LEAP-Seq n nonconfirming: | 17 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTTCTCATCCAATTCTGGGC |
| Suggested primer 2: | CGGTGTGTGTGTGTGTGTGT |