Insertion junction: LMJ.RY0402.085671_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.085671
Chromosome:chromosome 9
Location:630161
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre09.g403400 (1 of 3) PF13637//PF13920 - Ankyrin repeats (many copies) (Ank_4) // Zinc finger, C3HC4 type (RING finger) (zf-C3HC4_3) 5'UTR_intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GAAGAACCAGGTGGGGATCGAATGGCCGAC
Internal bar code:AGGCTCGCTCGTCCCTCCGAGT

Confirmation - LEAP-Seq

LEAP-Seq distance:765
LEAP-Seq percent confirming:98.0754
LEAP-Seq n confirming:6064
LEAP-Seq n nonconfirming:119
LEAP-Seq n unique pos:43

Suggested primers for confirmation by PCR

Suggested primer 1:GCTCACCTTAACCTGCTTGC
Suggested primer 2:GAAAGTCTTCTGCCACAGCC