| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.085686 |
| Chromosome: | chromosome 7 |
| Location: | 940662 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g319150 | PTP4 | Protein tyrosine phosphatase; (1 of 2) PTHR10367 - MRNA-CAPPING ENZYME | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CAAGGTGGGGGCAGGAGAAAGGGGGAGGAG |
| Internal bar code: | GCTTGGCCGGGCAGGCAGGACA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 464 |
| LEAP-Seq percent confirming: | 98.6487 |
| LEAP-Seq n confirming: | 73 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 13 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGGAAGTTGCTCTGTGCTTC |
| Suggested primer 2: | ATGTGGCACAGACCTAACCC |