| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.085725 |
| Chromosome: | chromosome_6 |
| Location: | 7001671 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre06.g296700 | HYDG1,HYDG | Hydrogenase assembly factor/biotin synthase | antisense | CDS |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | CGGCCTGTGCGGTGAGGAGCGCGGAAGGAG |
| Internal bar code: | TTGATAAAACATGTCCGAGTCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1041 |
| LEAP-Seq percent confirming: | 97.9764 |
| LEAP-Seq n confirming: | 9296 |
| LEAP-Seq n nonconfirming: | 192 |
| LEAP-Seq n unique pos: | 83 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAAGGTCAACCATTTGGCAG |
| Suggested primer 2: | CCAATCACTGCATGAACACC |