Insertion junction: LMJ.RY0402.085732_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.085732
Chromosome:chromosome 8
Location:455789
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre08.g358616 FAP398 Flagellar Associated Protein 398; (1 of 70) 3.1.3.16 - Protein-serine/threonine phosphatase / Serine/threonine specific protein phosphatase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTTCCATGTTATCCTTTCCGTTTGCTCCCA
Internal bar code:GCCTGGGGCTGTCCTTATCCCT

Confirmation - LEAP-Seq

LEAP-Seq distance:65
LEAP-Seq percent confirming:99.3976
LEAP-Seq n confirming:165
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:3

Suggested primers for confirmation by PCR

Suggested primer 1:TACCAACACACACCCATGCT
Suggested primer 2:ATGAACAGTGAGCCTCCCAC