| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.085761 |
| Chromosome: | chromosome 6 |
| Location: | 2689503 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g270350 | AMYB2,AMB2 | Beta-amylase 2; (1 of 3) 3.2.1.2 - Beta-amylase / Saccharogen amylase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCGTCGAGGCAGGTATATAACGGGAGCACG |
| Internal bar code: | CCCATTCAAGGCAGAAATGGGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 600 |
| LEAP-Seq percent confirming: | 98.9405 |
| LEAP-Seq n confirming: | 1214 |
| LEAP-Seq n nonconfirming: | 13 |
| LEAP-Seq n unique pos: | 11 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGTGAAGTAGATGTCGGGGT |
| Suggested primer 2: | CTAGAGGCCAGAAGCGATTG |