| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.085774 |
| Chromosome: | chromosome 17 |
| Location: | 1698962 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g708700 | HTR20,HTR27 | Histone H3; (1 of 35) K11253 - histone H3 (H3) | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AAGTTACTTTTGAGTTCTATATGAGACTTT |
| Internal bar code: | CCCAAGGCCTGAATGAAGATTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 180 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 156 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACACCGAGTTTGTTTGGAGG |
| Suggested primer 2: | GGGGTAGCACTAGCAAGCAG |