| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.085777 |
| Chromosome: | chromosome 17 |
| Location: | 5227450 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g735850 | (1 of 1) PF07303 - Occludin homology domain (Occludin_ELL) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGGATGCGTCAAGCTCAACCAAGTCACGCT |
| Internal bar code: | ATGAACGGGCGCAGTGATTTGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 427 |
| LEAP-Seq percent confirming: | 98.8927 |
| LEAP-Seq n confirming: | 1161 |
| LEAP-Seq n nonconfirming: | 13 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGTAAAGTCGCAGCTCCTTG |
| Suggested primer 2: | GGCCTGTCCAGGATGTATGT |