| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.085821 |
| Chromosome: | chromosome 12 |
| Location: | 5594000 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g531900 | FLVA,FLVA1 | (1 of 2) 1.5.3.22 - Coenzyme F420H(2) oxidase; Thylakoid flavodiiron protein | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCTGACGGGTGAGGGCTGGCTGCGCGAGTG |
| Internal bar code: | CGTTTATGGAGGTGGCCGTCTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 499 |
| LEAP-Seq percent confirming: | 99.0318 |
| LEAP-Seq n confirming: | 716 |
| LEAP-Seq n nonconfirming: | 7 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAGGCTTGAGGCTTTCATTG |
| Suggested primer 2: | CTGACGCACATCATCCTGAC |