| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.085827 |
| Chromosome: | chromosome 6 |
| Location: | 6209748 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g290550 | (1 of 1) PTHR23213//PTHR23213:SF243 - FORMIN-RELATED // SUBFAMILY NOT NAMED | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGTACGACGTGTCAGCTTTGCGTTACGGGC |
| Internal bar code: | TGGGGGCCTTGTGACTTGCTGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 445 |
| LEAP-Seq percent confirming: | 97.8218 |
| LEAP-Seq n confirming: | 3009 |
| LEAP-Seq n nonconfirming: | 67 |
| LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACAGGCTGCGAGGTAGAAAA |
| Suggested primer 2: | TTTTCTACCTCGCAGCCTGT |