Insertion junction: LMJ.RY0402.085830_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.085830
Chromosome:chromosome 6
Location:2795372
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre06.g271150 FAP74,C1d-HC3 (1 of 6) PF14874 - Flagellar-associated PapD-like (PapD-like); Flagellar Associated Protein 74 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGGAGGAACGGGACGGAATGTACTTAATGC
Internal bar code:TCCAGTCAGGGGGACCTCGCTG

Confirmation - LEAP-Seq

LEAP-Seq distance:492
LEAP-Seq percent confirming:99.1667
LEAP-Seq n confirming:357
LEAP-Seq n nonconfirming:3
LEAP-Seq n unique pos:2

Suggested primers for confirmation by PCR

Suggested primer 1:AAAAGACACCCATCGTCTGC
Suggested primer 2:GTATCCACTGCTGGTCGGTT