| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.085832 |
| Chromosome: | chromosome 2 |
| Location: | 1109354 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g081250 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTGCGAGTGGAGGTGGCAGTGCCCGGAGCG |
| Internal bar code: | ATGTTGCTGCTTAGCGAGTGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 302 |
| LEAP-Seq percent confirming: | 99.5192 |
| LEAP-Seq n confirming: | 1035 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCTGGGTTTCTGTATTGGGC |
| Suggested primer 2: | GGAATCACATGCGGAAAGTT |