| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.085846 |
| Chromosome: | chromosome 5 |
| Location: | 212560 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g234050 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCCTTCCTCATGGGTTGCAACAGCGATGTG |
| Internal bar code: | TGGTGCTGATTGGGACACTGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 718 |
| LEAP-Seq percent confirming: | 98.6713 |
| LEAP-Seq n confirming: | 2005 |
| LEAP-Seq n nonconfirming: | 27 |
| LEAP-Seq n unique pos: | 16 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCAAAGTCTGCAACGACTGC |
| Suggested primer 2: | ATGGAGTGTTGGCACACAAA |