| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.085853 |
| Chromosome: | chromosome 6 |
| Location: | 4051792 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g278209 | PRL8 | Predicted extracellular protein; (1 of 4) PTHR10334//PTHR10334:SF224 - CYSTEINE-RICH SECRETORY PROTEIN-RELATED // SUBFAMILY NOT NAMED | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTCGAAGTGCTAAACGGGGGCGCCAGCTCA |
| Internal bar code: | GGTTCTATCTTTTCAGTGGCAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 233 |
| LEAP-Seq percent confirming: | 99.4898 |
| LEAP-Seq n confirming: | 1365 |
| LEAP-Seq n nonconfirming: | 7 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGGCGTCTCTGCAATGTAAG |
| Suggested primer 2: | ATACAATGGTAAGGGCGCAG |