| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.085862 |
| Chromosome: | chromosome 13 |
| Location: | 1536372 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g572900 | THO3 | (1 of 1) 3.4.24.70 - Oligopeptidase A; Oligoendopeptidase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTCAAGTAGAGGTGCGGCCAGGACTGAGGG |
| Internal bar code: | GTGTGGCGTTTCTACCCAGGAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 521 |
| LEAP-Seq percent confirming: | 98.8439 |
| LEAP-Seq n confirming: | 171 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 12 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCAGTCCCTGCCTAGTCATC |
| Suggested primer 2: | GCAGGCTACATGGAGAGAGG |