| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.085869 |
| Chromosome: | chromosome 9 |
| Location: | 3842076 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g391875 | (1 of 1) PTHR22847//PTHR22847:SF453 - WD40 REPEAT PROTEIN // SUBFAMILY NOT NAMED | CDS|intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTTAAGCATATCCCCGCCGGCGCCCAAGAA |
| Internal bar code: | CTGTCAGCGCAGTTCCTCGGCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 183 |
| LEAP-Seq percent confirming: | 98.984 |
| LEAP-Seq n confirming: | 682 |
| LEAP-Seq n nonconfirming: | 7 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCAGGTTACGAAGCTGGAAG |
| Suggested primer 2: | CAGCACCCAATACACCCTCT |