| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.085881 |
| Chromosome: | chromosome 6 |
| Location: | 1755525 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g262050 | AEP1 | (1 of 1) 5.1.3.3 - Aldose 1-epimerase / Mutarotase; Aldose-1-epimerase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCCGCCCCTGCGCCGCACCTGGGGCTGCAG |
| Internal bar code: | GATGTGTCTTTCGCAAAGGGCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 231 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 50 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGTGTGATCGGCACTAGGAT |
| Suggested primer 2: | GACGCGGACTTGTAGGTAGC |