Insertion junction: LMJ.RY0402.085920_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.085920
Chromosome:chromosome 16
Location:2062805
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre16.g657250 GOX17,GOX19 Glyoxal oxidase 19; (1 of 12) 1.1.3.9 - Galactose oxidase / Beta-galactose oxidase CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CAAACTGTGTTGGGACTCAGCTCGCTGCTG
Internal bar code:CGCATATAACTTCCCCCTGGAC

Confirmation - LEAP-Seq

LEAP-Seq distance:256
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:22
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:5

Suggested primers for confirmation by PCR

Suggested primer 1:CGCATCAGCTCTCCATAACA
Suggested primer 2:AGGTAAATGACAACGGACGC