| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.085946 |
| Chromosome: | chromosome 10 |
| Location: | 4306730 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g450850 | (1 of 1) PF00168//PF16016 - C2 domain (C2) // Domain of unknown function (DUF4782) (DUF4782) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AAGGTTGTGGATAGGGCCCTCCCAGGGGGT |
| Internal bar code: | CGCGCCGCCGAGCGGCCACGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 740 |
| LEAP-Seq percent confirming: | 99.6637 |
| LEAP-Seq n confirming: | 16595 |
| LEAP-Seq n nonconfirming: | 56 |
| LEAP-Seq n unique pos: | 15 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAGACAGTAGCGTGGCACAA |
| Suggested primer 2: | CTCTCATCCCAATTCAGGGA |