Insertion junction: LMJ.RY0402.085946_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.085946
Chromosome:chromosome 10
Location:4306731
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre10.g450850 (1 of 1) PF00168//PF16016 - C2 domain (C2) // Domain of unknown function (DUF4782) (DUF4782) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CACCAAACCCAACCCCACTACAGTCGCACC
Internal bar code:TGTAGGACAGATCTACGCTCGG

Confirmation - LEAP-Seq

LEAP-Seq distance:581
LEAP-Seq percent confirming:98.323
LEAP-Seq n confirming:1583
LEAP-Seq n nonconfirming:27
LEAP-Seq n unique pos:12

Suggested primers for confirmation by PCR

Suggested primer 1:GAGACAGTAGCGTGGCACAA
Suggested primer 2:CTCTCATCCCAATTCAGGGA