| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.085946 |
| Chromosome: | chromosome_10 |
| Location: | 4306731 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre10.g450850 | sense | intron |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | CACCAAACCCAACCCCACTACAGTCGCACC |
| Internal bar code: | TGTAGGACAGATCTACGCTCGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 581 |
| LEAP-Seq percent confirming: | 98.323 |
| LEAP-Seq n confirming: | 1583 |
| LEAP-Seq n nonconfirming: | 27 |
| LEAP-Seq n unique pos: | 12 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAGACAGTAGCGTGGCACAA |
| Suggested primer 2: | CTCTCATCCCAATTCAGGGA |