| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.085951 |
| Chromosome: | chromosome 6 |
| Location: | 5848289 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g287900 | (1 of 1) PF10067 - Predicted membrane protein (DUF2306) (DUF2306) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CAGTATACCACAAGTCGAACACTGCCCAAT |
| Internal bar code: | GCAAGAATTTGGCAGTCTCTGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 867 |
| LEAP-Seq percent confirming: | 97.861 |
| LEAP-Seq n confirming: | 183 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCCAGTAGGGTGTCAGGTGT |
| Suggested primer 2: | GCGTCCCTTGTTTTGTGTTT |