| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.085958 |
| Chromosome: | chromosome 12 |
| Location: | 2424324 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g507051 | (1 of 2) K01193 - beta-fructofuranosidase (E3.2.1.26, sacA) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AACGAGTATCTGTCGAGCCGTGTACGTGTG |
| Internal bar code: | CGGTGCCCAGTCTTAACACCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 57 |
| LEAP-Seq percent confirming: | 94.8864 |
| LEAP-Seq n confirming: | 668 |
| LEAP-Seq n nonconfirming: | 36 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCGGGAGCAGTAGCAGTAAC |
| Suggested primer 2: | TACCAACTTCCCACTCTCCG |