Insertion junction: LMJ.RY0402.085960_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.085960
Chromosome:chromosome 11
Location:2719664
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre11.g477000 SNRK2D snRK1 family in Chlamydomonas, subgroup 2; (1 of 41) IPR000719//IPR011009 - Protein kinase domain // Protein kinase-like domain intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CGGTCGATGCCAGCATCCTCAAGGGTGTAC
Internal bar code:TACAAGTGGGCTACCCATAGGA

Confirmation - LEAP-Seq

LEAP-Seq distance:571
LEAP-Seq percent confirming:95.4356
LEAP-Seq n confirming:126789
LEAP-Seq n nonconfirming:6064
LEAP-Seq n unique pos:193

Suggested primers for confirmation by PCR

Suggested primer 1:CCTTGGACTGAAACGTGGTT
Suggested primer 2:TCCTCCTCGCTCTATTGCAT