| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.085969 |
| Chromosome: | chromosome_16 |
| Location: | 4968332 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre16.g685250 | SUB11 | antisense | intron |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | AGGAGTGACGCTGCGTCGGAGTCACCATCC |
| Internal bar code: | GGCAAGCGTCAGTACCATGGAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 597 |
| LEAP-Seq percent confirming: | 99.204 |
| LEAP-Seq n confirming: | 997 |
| LEAP-Seq n nonconfirming: | 8 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CACAGTCTTCCCGATTTGGT |
| Suggested primer 2: | TTTCAAGCATAGGGTTTGGC |