| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.085983 |
| Chromosome: | chromosome 1 |
| Location: | 3993606 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g025950 | PPR8 | (1 of 1) IPR000209//IPR002885 - Peptidase S8/S53 domain // Pentatricopeptide repeat; PentatricoPeptide Repeat protein 8 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGATGTGTGTGTGTGTGTGTGTGTATGTGG |
| Internal bar code: | GAGGACTGTTCGCACAAGGGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 458 |
| LEAP-Seq percent confirming: | 98.7099 |
| LEAP-Seq n confirming: | 2678 |
| LEAP-Seq n nonconfirming: | 35 |
| LEAP-Seq n unique pos: | 20 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GACCTGGGCTTCAAGGTGTA |
| Suggested primer 2: | CGTCAGCACCTTTGAAGTGA |