| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.085992 |
| Chromosome: | chromosome 16 |
| Location: | 1691815 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g654700 | PHC61 | Pherophorin-chlamydomonas homolog 61; (1 of 71) PF12499 - Pherophorin (DUF3707) | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTCGTAGTGGTGATAGTCACATCGCCTGTA |
| Internal bar code: | TCGAAGCCAGGGTCTGGTTTGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 671 |
| LEAP-Seq percent confirming: | 98.684 |
| LEAP-Seq n confirming: | 5474 |
| LEAP-Seq n nonconfirming: | 73 |
| LEAP-Seq n unique pos: | 42 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCATAGAGCGAAGCCTTGTC |
| Suggested primer 2: | GCCAAGGTTGAATGACCTGT |