Insertion cassette: | CIB1 |
Side of cassette: | 5' |
Strand: | - |
Strain: | LMJ.RY0402.085994 |
Chromosome: | chromosome 10 |
Location: | 6286101 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre10.g465000 | (1 of 8) 2.7.12.2 - Mitogen-activated protein kinase kinase / MKK | intron |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATCCCCAAATAACCCCCATGCTCCTCGCCT |
Internal bar code: | CCGTTGGGCGGCTTTCTCCGTC |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 1137 |
LEAP-Seq percent confirming: | 99.5569 |
LEAP-Seq n confirming: | 4718 |
LEAP-Seq n nonconfirming: | 21 |
LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | CAGAAAGAGGTCGCGTTAGG |
Suggested primer 2: | AGTGTACACACGCGAGAACG |