Insertion junction: LMJ.RY0402.085994_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.085994
Chromosome:chromosome 10
Location:6286101
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre10.g465000 (1 of 8) 2.7.12.2 - Mitogen-activated protein kinase kinase / MKK intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ATCCCCAAATAACCCCCATGCTCCTCGCCT
Internal bar code:CCGTTGGGCGGCTTTCTCCGTC

Confirmation - LEAP-Seq

LEAP-Seq distance:1137
LEAP-Seq percent confirming:99.5569
LEAP-Seq n confirming:4718
LEAP-Seq n nonconfirming:21
LEAP-Seq n unique pos:8

Suggested primers for confirmation by PCR

Suggested primer 1:CAGAAAGAGGTCGCGTTAGG
Suggested primer 2:AGTGTACACACGCGAGAACG