| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.085995 |
| Chromosome: | chromosome 10 |
| Location: | 1879602 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g431650 | (1 of 2) 2.7.1.86 - NADH kinase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCAGCAACGCAGTCCGGCGCCCACACCCAA |
| Internal bar code: | CCCAAGCCGGACGATGGAGCAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 143 |
| LEAP-Seq percent confirming: | 99.7368 |
| LEAP-Seq n confirming: | 1516 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGCACCAGAAGTGGTGAGTT |
| Suggested primer 2: | GTGCTGTGGGCAATAAACCT |