| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.086008 |
| Chromosome: | chromosome 10 |
| Location: | 4389735 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g451450 | (1 of 66) 2.7.12.1 - Dual-specificity kinase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTGCCCCGACGCGGCGCCGCTAGGCCGGCC |
| Internal bar code: | ACCACCGGTGATTCTTTCATCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 256 |
| LEAP-Seq percent confirming: | 99.5057 |
| LEAP-Seq n confirming: | 4026 |
| LEAP-Seq n nonconfirming: | 20 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCACCTGAGCGAGATAGTCC |
| Suggested primer 2: | GTAATTGGCGACGAGCAAGT |