| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.086010 |
| Chromosome: | chromosome 3 |
| Location: | 5344836 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g184151 | NAT9 | N-acetyltransferase; (1 of 1) PTHR13256//PTHR13256:SF16 - N-ACETYLTRANSFERASE 9 // N-ACETYLTRANSFERASE 9 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AACTTCATCACTTCTACGAATGCCTCCGGG |
| Internal bar code: | TTTCTCATGGACTGTCAGTTTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1000 |
| LEAP-Seq percent confirming: | 99.3333 |
| LEAP-Seq n confirming: | 149 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTGGCCAAGATACACGAGGT |
| Suggested primer 2: | GTGCATACCTTTGGGCTTGT |