Insertion junction: LMJ.RY0402.086012_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.086012
Chromosome:chromosome 12
Location:8824494
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre12.g545101 XDH1 (1 of 1) 1.17.1.4 - Xanthine dehydrogenase / Xanthine/NAD(+) oxidoreductase; Xanthine dehydrogenase/oxidase CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CGATGTTGCCGCCCACAGCCGACACGTTGC
Internal bar code:CTGCGTCATATTATTTCCTAAC

Confirmation - LEAP-Seq

LEAP-Seq distance:517
LEAP-Seq percent confirming:99.8438
LEAP-Seq n confirming:1278
LEAP-Seq n nonconfirming:2
LEAP-Seq n unique pos:8

Suggested primers for confirmation by PCR

Suggested primer 1:GTGGGCATCGAGATGAAGTT
Suggested primer 2:TGCAGAGTGGTATTGTCCCA