Insertion junction: LMJ.RY0402.086019_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.086019
Chromosome:chromosome_10
Location:1649804
Confidence (%):95
Locus disrupted Locus common name Defline Orientation Feature
Cre10.g429750 antisense intron

Insertion site details

Flanking sequence (orientation from cassette outwards):TTTGATGCCGCACCACACCGCACCACACCA
Internal bar code:TTCGGTCGCGACGAAGCCATCT

Confirmation - LEAP-Seq

LEAP-Seq distance:656
LEAP-Seq percent confirming:99.7046
LEAP-Seq n confirming:2025
LEAP-Seq n nonconfirming:6
LEAP-Seq n unique pos:10

Suggested primers for confirmation by PCR

Suggested primer 1:TGCTGTGCATGTACCTGTGA
Suggested primer 2:GAGGCTTTGATGTGTTGGGT