| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.086021 |
| Chromosome: | chromosome 4 |
| Location: | 2436765 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g221450 | PHC54 | Putative pherophorin-chlamydomonas homolog; (1 of 71) PF12499 - Pherophorin (DUF3707) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCGCACGCGCACATGCATCGCCACATAGAT |
| Internal bar code: | ATGGCCCGACTTGTTCGTCATC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 896 |
| LEAP-Seq percent confirming: | 95.9131 |
| LEAP-Seq n confirming: | 8472 |
| LEAP-Seq n nonconfirming: | 361 |
| LEAP-Seq n unique pos: | 13 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAGGCGTGATTGTTAGCAGA |
| Suggested primer 2: | CGTAGGATGGACGGACTTGT |