Insertion junction: LMJ.RY0402.086027_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.086027
Chromosome:chromosome 6
Location:3821318
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre06.g278169 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CACCACCCGCCCTGGCAGTACTCGCTGCCT
Internal bar code:GCGGTCATCACGGCGAACAGAC

Confirmation - LEAP-Seq

LEAP-Seq distance:774
LEAP-Seq percent confirming:90.2207
LEAP-Seq n confirming:1799
LEAP-Seq n nonconfirming:195
LEAP-Seq n unique pos:11

Suggested primers for confirmation by PCR

Suggested primer 1:ATTTTCGCACATCCAACTCC
Suggested primer 2:TAATCTTTTGGTTCCCGCTG