Insertion junction: LMJ.RY0402.086029_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.086029
Chromosome:chromosome 12
Location:4857076
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre12.g525050 SMC6A (1 of 2) PTHR19306//PTHR19306:SF6 - STRUCTURAL MAINTENANCE OF CHROMOSOMES 5,6 SMC5, SMC6 // PROTEIN C23H4.6, ISOFORM A-RELATED; Structural Maintenance of Chromosomes protein intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTCGAACAGCTTCATAGCCTCGTCCACCAG
Internal bar code:AGCCGCTCGTTAGTACACAACT

Confirmation - LEAP-Seq

LEAP-Seq distance:567
LEAP-Seq percent confirming:99.5
LEAP-Seq n confirming:1791
LEAP-Seq n nonconfirming:9
LEAP-Seq n unique pos:2

Suggested primers for confirmation by PCR

Suggested primer 1:ATGTGTGTGCGTCTGGGTTA
Suggested primer 2:TCCGTCACACTGATGTAGGC