| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.086093 |
| Chromosome: | chromosome 3 |
| Location: | 6316022 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g193450 | (1 of 29) PF00612 - IQ calmodulin-binding motif (IQ) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTCGCACCTCACCACACCTCTCCACAGCAC |
| Internal bar code: | GTCTCTATCTCCCCGTGGCTTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 216 |
| LEAP-Seq percent confirming: | 99.9271 |
| LEAP-Seq n confirming: | 1370 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCTCCACACGCACTTGTTC |
| Suggested primer 2: | TGAACAAACTGCAGCCTGAC |