| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.086099 |
| Chromosome: | chromosome 3 |
| Location: | 1979834 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g155800 | (1 of 1) K15025 - probable RNA-binding protein EIF1AD (EIF1AD) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCCGATATCACCCTTTGACAGAATACTCCA |
| Internal bar code: | ACGTCACGTACGTAAATTCGTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 290 |
| LEAP-Seq percent confirming: | 98.5883 |
| LEAP-Seq n confirming: | 4330 |
| LEAP-Seq n nonconfirming: | 62 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCAGATGGTGCAAGTGCTTC |
| Suggested primer 2: | CGCAGCTATCGTATCGTTGA |