| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.086100 |
| Chromosome: | chromosome 2 |
| Location: | 7912770 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g141250 | PPP10 | (1 of 2) K14497 - protein phosphatase 2C (PP2C); Phosphoprotein phosphatase 2C-related | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGGGTGCACGTGAAGCCGCGGGCCGATGGC |
| Internal bar code: | CCATAGGTTAGCATACCTGGCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1300 |
| LEAP-Seq percent confirming: | 97.97 |
| LEAP-Seq n confirming: | 2413 |
| LEAP-Seq n nonconfirming: | 50 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCTGTTTGCTCTAAGCGGGT |
| Suggested primer 2: | GTGGTGTGCAGGATGTGTTC |