| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.086104 |
| Chromosome: | chromosome 2 |
| Location: | 1868972 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g087551 | (1 of 1) K17600 - vacuolar protein sorting-associated protein 54 (VPS54) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTCTGCTCCTCCTGGCCCCAGCCTCCCAAC |
| Internal bar code: | GGCCCTCAGTTTTGGACGTTCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 214 |
| LEAP-Seq percent confirming: | 84.9057 |
| LEAP-Seq n confirming: | 45 |
| LEAP-Seq n nonconfirming: | 8 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGTGGTAAACAACACGTTCG |
| Suggested primer 2: | GTGGGATGGAGGTGAGCTT |