| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.086108 |
| Chromosome: | chromosome 2 |
| Location: | 1756426 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g086500 | PGM10 | (1 of 2) PTHR23029//PTHR23029:SF24 - PHOSPHOGLYCERATE MUTASE // SUBFAMILY NOT NAMED; Phosphoglycerate mutase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGAACTACGGCCTGTCACGGACTCACACCC |
| Internal bar code: | GGTGTGGATTCTGTGGGAGCGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 697 |
| LEAP-Seq percent confirming: | 99.2841 |
| LEAP-Seq n confirming: | 3051 |
| LEAP-Seq n nonconfirming: | 22 |
| LEAP-Seq n unique pos: | 24 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTGGCTTCTTCTTCGTTCGT |
| Suggested primer 2: | ACCAACAGATACCTCACGCC |