| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.086113 |
| Chromosome: | chromosome 13 |
| Location: | 1275527 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g570851 | PSB34 | PSII assembly protein; (1 of 1) PTHR35753//PTHR35753:SF1 - FAMILY NOT NAMED // PROLINE-RICH FAMILY PROTEIN | 5'UTR/5'UTR_intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTGGCCCGCAGTGTCCTTCTCCACATAGCC |
| Internal bar code: | GGGCGTCACCTGTGTGAGAGAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 866 |
| LEAP-Seq percent confirming: | 97.8729 |
| LEAP-Seq n confirming: | 3635 |
| LEAP-Seq n nonconfirming: | 79 |
| LEAP-Seq n unique pos: | 29 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGTTGAAGTTGTCCTGCTGC |
| Suggested primer 2: | GGCTCCGGTGTGCTATGTAT |