| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.086116 |
| Chromosome: | chromosome 10 |
| Location: | 4892604 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g454650 | RPC5 | (1 of 1) K14721 - DNA-directed RNA polymerase III subunit RPC5 (RPC5, POLR3E); DNA-directed RNA polymerase III, 8 kD | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTCGCCAACCTGCCTAAACCCTGCCCCATC |
| Internal bar code: | CCCGGGGATATGGGTAAGGCCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 687 |
| LEAP-Seq percent confirming: | 99.2811 |
| LEAP-Seq n confirming: | 5662 |
| LEAP-Seq n nonconfirming: | 41 |
| LEAP-Seq n unique pos: | 21 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTTTCAATGTCGTGGTGGTG |
| Suggested primer 2: | AATTCTGCCTTGTGGATTGG |