| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.086139 |
| Chromosome: | chromosome 16 |
| Location: | 5626565 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g679650 | (1 of 1) PTHR19961:SF18 - FI19014P1 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTGTAGACTGTGTTGAAGAGCACAAGGAAA |
| Internal bar code: | AACAGTCCCCAGGCATGACAGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 573 |
| LEAP-Seq percent confirming: | 93.92 |
| LEAP-Seq n confirming: | 12327 |
| LEAP-Seq n nonconfirming: | 798 |
| LEAP-Seq n unique pos: | 55 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCCACAGCGTTACTAGCCTC |
| Suggested primer 2: | TTTGGTTTAGTTTGGGCTGG |