Insertion junction: LMJ.RY0402.086140_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.086140
Chromosome:chromosome 16
Location:3638945
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre16.g686285 (1 of 21) IPR011016 - Zinc finger, RING-CH-type 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TTCGCTCTGCACCAGGCAGCTGCGCACTCG
Internal bar code:GTAGGAAACGAAGGGCGTTGTG

Confirmation - LEAP-Seq

LEAP-Seq distance:704
LEAP-Seq percent confirming:99.2721
LEAP-Seq n confirming:2864
LEAP-Seq n nonconfirming:21
LEAP-Seq n unique pos:13

Suggested primers for confirmation by PCR

Suggested primer 1:CTTCAATTCTTCCCTGCTGC
Suggested primer 2:CGGGGGATGTGTCATTTATC