| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.086152 |
| Chromosome: | chromosome 2 |
| Location: | 6548929 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g116950 | HDA1 | RPD3/HDA1 type histone deacetylase; (1 of 2) 3.5.1.4 - Amidase / Acylase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CATGGTGTTAAATGGCTTTACTTTCACGAC |
| Internal bar code: | TCCCAGGCCTATACGGGGATGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 476 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 131 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGTTACAGCGAGCTCTACGG |
| Suggested primer 2: | CCAAAACCTTATAACGGCGA |