| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.086178 |
| Chromosome: | chromosome 10 |
| Location: | 2130001 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g433476 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATCGATGCGAGTGTCAGGTTTCAAGAATGT |
| Internal bar code: | AGCCATGTCGTCAATCGGTATC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 656 |
| LEAP-Seq percent confirming: | 98.9256 |
| LEAP-Seq n confirming: | 2394 |
| LEAP-Seq n nonconfirming: | 26 |
| LEAP-Seq n unique pos: | 27 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTTGCATGCATTTTCGACAT |
| Suggested primer 2: | GTGCCATTGTGCTCTGCTAA |