| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.086183 |
| Chromosome: | chromosome 5 |
| Location: | 815535 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g248100 | ANK1 | Predicted protein with ankyrin repeats; (1 of 78) IPR002110//IPR020683 - Ankyrin repeat // Ankyrin repeat-containing domain | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AATTGAGCAGCAGCGGCTTCCAGATACGTG |
| Internal bar code: | TAAAGTCCGCTTTAGCGGTAGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1164 |
| LEAP-Seq percent confirming: | 99.6408 |
| LEAP-Seq n confirming: | 14425 |
| LEAP-Seq n nonconfirming: | 52 |
| LEAP-Seq n unique pos: | 42 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTCGGCTCAGTAAACGGAAG |
| Suggested primer 2: | TCAGCTGTGCTGTCGGTAAC |