| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.086212 |
| Chromosome: | chromosome 9 |
| Location: | 1632393 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g397200 | CCT6 | T-complex protein 1, zeta subunit | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCCAGCCCGTACGGAGGGTGCCGAAGGAGT |
| Internal bar code: | CCTCGCCTGGGACGACTGGATG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 23 |
| LEAP-Seq percent confirming: | 89.1892 |
| LEAP-Seq n confirming: | 99 |
| LEAP-Seq n nonconfirming: | 12 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATCAACCATCCCTCTTGACG |
| Suggested primer 2: | GTGGTTTTGAGAGGGCACAT |