Insertion junction: LMJ.RY0402.086232_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.086232
Chromosome:chromosome_9
Location:3915434
Confidence (%):73
Locus systematic id Locus common name Defline Orientation Feature
Cre09.g392282 DHC2 Dynein heavy chain 2, inner arm dynein d (monomeric) antisense CDS

Insertion site details

Flanking sequence (orientation from cassette outwards):TCATCGTCATCCGGGAATTCGCGCTTGAGG
Internal bar code:GGTTCGTGCGCGGAACACGGCA

Confirmation - LEAP-Seq

LEAP-Seq distance:350
LEAP-Seq percent confirming:98.8903
LEAP-Seq n confirming:1515
LEAP-Seq n nonconfirming:17
LEAP-Seq n unique pos:9

Suggested primers for confirmation by PCR

Suggested primer 1:CAGCTTCTTGTTGTCGTCCA
Suggested primer 2:TACCGTAGCAACCATAGCCC